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Centre Testing International Co., Ltd. (CTI) is the pioneer and leader in the TIC Industry which provides one-stop solutions on testing, inspection, certification, calibration, audit, training & technical services.

 
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Composition Analysis of Cell Culture Media/Fluids

The main components of cell culture medium/fluid include inorganic salts, trace elements, amino acids, vitamins, organic acids, nucleosides, purines, etc. CTI Sinotest provides you with quantitative analysis of 30 trace elements and 60 organic compounds, and semi-quantitative scanning analysis of more than 60 elements and 100 organic compounds.

Composition Analysis of Cell Culture Media/Fluids

Service Background

The composition of cell culture medium/fluid is critical to cell growth. Monitoring changes in key components of the culture medium is an important part of the biopharmaceutical process.

 

Applicable Products

Applicable to synthetic cell culture media/fluids.
 

Service Content

 

 

Semi-quantitative scanning analysis

Semi-quantitative scanning of elements in culture media by ICP-MS to quickly determine the types and range of trace elements. Semi-quantitative scanning analysis of organic matter in culture medium by LC-MSMS method, choosing analogues as internal standards.

  

 

Quantitative analysis

ICP-OES was used to quantitatively analyze the inorganic salts with high content in the medium; ICP-MS was used to quantitatively analyze the trace elements with low content in the medium; and LC-QQQ was used to quantitatively analyze the target substances. The methodological validation meets the relevant requirements of ICH Q2 R2 guidelines.

 

Featured Services

Detection of about 30 kinds of inorganic salts and trace elements.

Category

Serial number

Element symbol

Testing instrument

Inorganic Salts

1

Na

ICP-OES/MS

2

K

ICP-OES/MS

3

Ca

ICP-OES/MS

4

Mg

ICP-OES/MS

5

P

ICP-OES/MS

6

S

ICP-OES/MS

Trace Elements

1

Fe

ICP-OES/MS

2

Co

ICP- MS

3

Ni

ICP- MS

4

Cu

ICP- MS

5

Zn

ICP- MS

6

Se

ICP- MS

7

Cr

ICP- MS

8

Mn

ICP- MS

9

Mo

ICP- MS

10

B

ICP- MS

11

Li

ICP- MS

12

Al

ICP- MS

13

Si

ICP- MS

14

V

ICP- MS

15

Sn

ICP- MS

16

Ge

ICP- MS

17

Sr

ICP- MS

18

Rb

ICP- MS

19

Ba

ICP- MS

20

Zr

ICP- MS

21

Pb

ICP- MS

22

Ag

ICP- MS

23

Ti

ICP- MS

24

Cd

ICP- MS

25

As

ICP- MS

 

Detects 24 amino acids, including 10 essential amino acids and 14 non-essential amino acids for cell growth.

Classification

No.

Name

Abbreviation

Essential Amino Acids

1

L-Isoleucine

Iso

2

L-Leucine

Leu

3

L-Lysine

Lys

4

L-Methionine

Met

5

L-Phenylalanine

Phe

6

L-Threonine

Phe

7

L-Tryptophan

Try

8

L-Valine

Val

9

L-Histidine

His

10

L-Glutamine

Gln

Nonessential Amino Acids

1

L-Alanine

Ala

2

L-Arginine

Arg

3

L-Aspartic acid

Asp

4

L-Cystine

Cys-cys

5

L-Glutamic acid

Glu

6

Glycinev

Gly

7

L-Proline

Pro

8

L-Serine

Ser

9

L-Tyrosine

Tyr

10

L-Cysteine

Cys

11

L-Asparagine

Asn

12

Taurine

Tau

13

L-Ornithine

Orn

14

Hydroxy-L-proline

HyPro
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